Vol. 7, Issue 2, Part B (2025)

The research explores the phytochemical profile of Hygrophila auriculata leaf extract through the application of solvents with differing polarities, aiming to uncover biologically active constituents with therapeutic potential. Leaves of Hygrophila auriculata were subjected to extraction using solvents ranging from non-polar to highly polar namely petroleum ether (low polarity), chloroform, ethyl acetate, ethanol, and water (high polarity). Phytochemical screening identified a diverse array of bioactive compounds namely alkaloids, flavonoids, saponins, phenolics, tannins, glycosides, and terpenoids whose relative abundance varied depending on the solvent used during extraction. The ethanol extract demonstrated the most comprehensive array of phytochemicals, particularly rich in phenolic compounds, tannins, alkaloids, carbohydrates, terpenoids, and flavonoids compounds widely recognized for their antioxidant capabilities. These findings affirm that Hygrophila auriculata harbors a diverse set of bioactive agents potentially beneficial for managing various health conditions, including infertility. Continued investigation, particularly focusing on the isolation and structural elucidation of individual compounds, is recommended to further evaluate their pharmacological relevance.