Vol. 7, Issue 2, Part B (2025)

The present study focuses on the formulation and evaluation of an antidiabetic Churna prepared using Guava leaves (Psidium guajava), Fenugreek (Trigonella foenum-graecum), Ginger (Zingiber officinale), Neem (Azadirachta indica), and Cardamom (Elettaria cardamomum). The Churna was standardized through organoleptic assessment, physicochemical analysis, micromeritic evaluation, heavy metal testing, and phytochemical screening. The formulation exhibited acceptable quality parameters, including neutral pH, low moisture content, and the absence of foreign organic matter and toxic heavy metals. Phytochemical analysis confirmed the presence of carbohydrates, tannins, flavonoids, phenols, alkaloids, steroids, glycosides, and proteins, indicating a rich profile of bioactive constituents. In vitro antidiabetic activity was evaluated using α-amylase and α-glucosidase inhibition assays, where the Churna demonstrated significant dose-dependent inhibitory activity. The IC₅₀ values of the Churna (46.36 µg/mL for α-amylase and 39.92 µg/mL for α-glucosidase) were comparable to those of the standard drug acarbose. These findings suggest that the formulated antidiabetic Churna possesses promising antidiabetic potential and could serve as a natural therapeutic option for controlling postprandial hyperglycemia.